S7973-97A
Grade
PurifiedConjugate
HRPSpecificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase and anti-streptavidin. No reaction was observed against anti-avidin.Applications
E IHC WBEU Commodity Code
38220090Shipping Temp
Blue IceStorage Temp
-20°CStreptavidin (HRP)
Streptavidin is a non-glycosylated polypeptide chain having a molecular mass of 240kD and containg 4 subunits each with a binding site for biotin. The neutral isoelectric point of Streptavidin and the high affinity of the biotin-streptavidin interaction result in a very quick reaction with a high signal to noise ratio. Streptavidin does not react non-specifically with endogenous lectins when used in immunological assays on cells and tissues. Streptavidin is non- or very low charged around pH 7, reducing NSB to charged molecules to a very limited level.
Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:20,000-1:200,000 Western Blot: 1:10,000-1:40,000 Immunohistochemistry: 1:1000-1:5000 Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Source
Streptomyces avidinii
Concentration
~1mg/ml (UV 280)
Form
Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease Free), 0.01% gentamicin sulfate. Labeled with horseradish peroxidase (HRP).
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase and anti-streptavidin. No reaction was observed against anti-avidin.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
