Technical Data

S8022-03H
Grade
Purified
Accession Number
000338
Molecular Weight
38
EU Commodity Code
30021019
Shipping Temp
Dry Ice
Storage Temp
-70°C
BSA Free
Yes
Notes
BSA Free
SULT1C2, Recombinant, Human, Carrier Free (Sulfotransferase 1C2, ST1C2, Sulfotransferase 1C1, SULT1C#1, humSULTC2, SULT1C1)

Cytosolic sulfotransferases catalyze the sulfonation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. They are distinct from Golgi-resident sulfotransferases by the absence of transmembrane domains and are located in the cytoplasm. SULT1C2 is mainly expressed in the gastrointestinal tract stomach, duodenum, jejunum, ileum, colon, caecum and rectum), liver and kidneys, but not in the lungs. In contrast, SULT1C4, a sulfotransferase that is most closely related to SULT1C2, is expressed at higher levels in fetal lung and kidney and at lower levels in fetal heart. So far, SULT1C2 is found to be active only on pnitrophenol. The enzymatic activity of our recombinant human SULT1C2 was determined using a phosphatase coupled assay.

Source
Recombinant corresponding to aa2-296 from human SULT1C2 fused to His-tag at N-terminal expressed in E.coli.
Molecular Weight
~38kD
Biological Activity
Measured by its ability to transfer sulfate from PAPS to 4nitrophenol. The specific activity is >45pmol/min/ug, as measured under the described conditions.
Endotoxin: ~1EU/1ug (LAL)
Storage and Stability
Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Source
E. coli
Purity
~80% (SDS-PAGE)
Concentration
As reported
Form
Supplied as a liquid in MES, sodium chloride, DTT. BSA free.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Falany, C. N. (1997) FASEB J. 11:206. 2. Gamage, N. U. et al. (2006) Toxicol. Sci. 90:5. 3. Hehonah, N. et al. (1999) Int J. Biochem. Cell. Biol. 31:869. 4. Sakakibara, Y. et al. (1998) J. Biol. Chem. 273:33929. 5. Prather, B. et al. (2012) Anal. Biochem. 423:86.
USBio References
No references available
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