Technical Data

T2030
Grade
Affinity Purified
EU Commodity Code
38220090
Shipping Temp
Blue Ice
Storage Temp
-20°C
TCF Reporter Plasmid (TOPflash, TK-luciferase reporter)

Transfection grade T-cell factor (TCF) reporter plasmid containing two sets (with the second set in the reverse orientation) of three copies of the TCF binding site (wild type) upstream of the Thymidine Kinase (TK) minimal promoter and Luciferase open reading frame. FOPflash (F5950) containing mutated TCF binding sites is also available as a negative control.

Size
5.5kb
Applications
Suitable for use in TCF assays. Other applications not tested.
Recommeded Dilutions
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity
Purified by immunoaffinity chromatography.
Concentration
As Reported
Form
Supplied as a liquid in 10mM Tris-HCl, pH 8.0, 1mM EDTA.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
, 1. Coghlan, M.P., et al, Chem. Biol. 10: 793-803, 2000. 2. Ishitani, T., et al., Nature 399: 798-800, 1999. 3. Korinek, V., et al., Mol. Cell. Biol. 18: 1248-1256, 1998.
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net