Zinc is an essential nutrient for all organisms because of the many important roles this metal plays. Movement of zinc into and out of cells and subcellular organelles is mediated by zinc transporter proteins. In many organisms, zinc uptake is mediated by members of the ZIP family of metal ion transporters. In mammals, the Zip1, Zip2, Zip3, Zip4, Zip5, LIV- 1 (Zip6), KE4 (Zip7), and BIGM103 (Zip8) proteins have been implicated in zinc uptake in a variety of cell and tissue types.

ZIP4 rat: 656aa; human: 647aa; mouse: 660aa - 71kD; Chromosome: 15D3. It is highly expressed in the small intestine and embryonic visceral yolk sac; weakly expressed in the stomach and liver. Found to the apical surface of enterocytes and visceral endoderm cells during zinc deficiency.

Synthetic peptide corresponding to 20aa of human ZIP4 (Accession #Q6P5W5), C-terminal, Cytoplasmic domain.

~3kD

Suitable for use in ELISA and Antibody Blocking. Not suitable for Western Blot. Other applications not tested.

ELISA: 5-10ug control peptide per 1ug of aff pure IgG or 1ul antiserum Optimal dilutions to be determined by the researcher.

Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Synthetic peptide

Purified

~1mg/ml

Supplied as a lyophilized powder from PBS, 0.05% sodium azide.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.