Zymolyase, produced by a submerged culture of Arthrobacter luteus (1), is an enzyme preparation which effectively lyses cell walls of viable yeast cells. An essential enzyme responsible for lysis of viable yeast cells in this preparation is b-1, 3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with b-1,3-linkages and releases laminaripentaose specifically as the main and minimum product unit (4,5,10,11). This lytic activity releases spheroplasts and protoplasts in the preparation of yeast DNA prior to restriction enzyme digestion and Southern Blot analysis. The extent of lysis of yeast cells by Zymolyase varies with yeast strain, growth stage of yeast and cultural condition (6-8).
| USBio Catalog # | Product Name |
|---|---|
| Z1000 | Zymolyase 20T (Lyticase, Yeast Lytic Enzyme) CAS: 37340-57-1 |
| Z1001 | Zymolyase 20T Concentrate 50mg/ml, 0.1M Sorbitol (Lyticase, Yeast Lytic Enzyme) CAS: 37340-57-1 |
| Z1004 | Zymolyase 100T (Lyticase, Yeast Lytic Enzyme) CAS: 37340-57-1 |
| Z1005 | Zymolyase 100T Concentrate 10mg/ml, 0.1M Sorbitol (Lyticase, Yeast Lytic Enzyme) CAS: 37340-57-1 |
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