Protocols

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Protocols

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Media Formulations

Molecular Biology

PCR

A

Alkaline Lysis Buffers A, B, C

C

Cell Culture Protocols

Cell Staining Buffer

Cell Stimulation Cocktail

Culture Conditions and Types of Growth Media for Mammalian Cells

V

B

Bouin's Fixative

E

Ehrlich's Solution

F

G

Gentian Violet Stain

H

Hollande's Fixative

I

Intracellular Antigen Staining

K

Kovacs' Reagent

P

Papanicolaou's Stain

Paraformaldehyde 2%

S

Schiff's Reagent

T

Tissue Fixative Solution

Tissue Lysates Preparation

Z

Zenker's Fixative

A

A Broth (Powder)

Amies Broth with Charcoal (Amies Transport Medium) (Powder)

Antibiotic Medium #1 (Powder)

Antibiotic Medium #3 (Powder)

Antibiotic Medium #4 (Powder)

Antibiotic Medium #9 (Powder)

Antibiotic Medium #10 (Powder)

Antibiotic Medium #11 (Powder)

APT (All Purpose Tween) Agar (Powder)

Azide Dextrose Broth (Powder)

B

Bacillus Cereus Medium (BCM) (Powder)

Baird Parker Agar (Powder)

BiGGY Agar (Powder)

Bile Esculin Agar (Powder)

Bile Esculin Azide Agar (Powder)

Bismuth Sulfite Agar (Powder)

Blood Agar Base No. 2 (Powder)

Blood Agar Base, Low pH (Powder)

Blood Agar Base, pH 7.4 (Powder)

Brain Heart Infusion Agar (Powder)

Brain Heart Infusion Broth (Powder)

Brain Heart Infusion Broth w/o Dextrose (Powder)

Brilliant Green Agar (Powder)

Brilliant Green Agar w/Sulfadiazine (Powder)

Brilliant Green Bile Broth 2% (Powder)

Bromthymol Blue

Brucella Agar (Powder)

Brucella Broth (Powder)

Buffered Peptone Water (Powder)

C

Campy Selective Agar Base (Powder)

Casman Medium Base (Powder)

Cetrimide Agar, Non-USP (Pseudosel Agar®) (Powder)

Chapman Stone Medium (Powder)

CLED Agar (Powder)

CLED Agar, Bevis (Powder)

Clostridium Difficile Agar (Powder)

Clostrisel Agar (Powder)

Columbia Blood Agar Base (Powder)

Columbia CNA Agar (Powder)

Cooked Meat Medium (Powder)

Corn Meal Agar (Powder)

D

Decarboxylase Broth Moeller (Powder)

D/E Neutralizing Agar (Powder)

Deoxycholate Agar (Powder)

Deoxycholate Citrate Agar (Powder)

Deoxycholate Citrate Lactose Sucrose (DCLS) Agar (Powder)

Deoxycholate Lactose Agar (Powder)

Dermatophyte Test Medium (DTM Test Agar) (Powder)

Dextrose Agar (Powder)

Dextrose Broth (Powder)

Dextrose Phosphate Broth (Powder)

Dextrose Starch Agar (Powder)

Dextrose Tryptone Agar (Powder)

Dextrose Tryptone Broth (Powder)

Deoxyribonuclease (DNase) Test Agar (Powder)

Deoxyribonuclease (DNase) Test Agar w/Toludine Blue (Powder)

E

Earle’s Balanced Salts (Powder)

EC Medium (Powder)

EC Medium w/MUG (Powder)

Elliker Broth (Powder)

Eosin Methylene Blue Agar (Powder)

Eosin Methylene Blue Agar, Levine (Powder)

Eugonic Broth (Powder)

F

Flow Cytometry General Protocol

Formaldehyde Gel Running Buffer

Fluid Thioglycollate Medium w/K Agar (Powder)

G

Glycerol Tolerant Gel Buffer 20X

H

H Broth (Powder)

Hanks Balanced Salts (10X) (Powder)

Hollande's Fixative

L

L-Broth (Luria Broth) (Powder)

Lambda Agar (Powder)

Lambda Broth (Powder)

LB Agar Lennox (Powder)

LB Agar Lennox, Animal Free (Powder) (Lennox L agar)

LB Agar Miller (Powder)

LB Agar Miller, Animal Free (Powder) (Miller's LB agar, Luria-Bertani agar)

LB Broth Lennox (Powder)

LB Broth Lennox, Animal Free (Powder) (Lennox L broth base)

LB Broth Miller (Powder)

LB Broth Miller, Animal Free (Powder) (Miller's LB broth, Luria-Bertani broth)

LPGA Agar Medium

M

M63 Medium (Powder)

M9 Minimal Salts (Powder)

N

Nutrient Agar (Powder)

Nutrient Agar 1.5% (Powder)

Nutrient Broth (Powder)

NZ Broth (Powder)

NZC Broth (Powder)

NZCYM Agar (Powder)

NZM Agar (Powder)

NZM Broth (Powder)

NZYDT Broth (Powder)

NZYM Agar (Powder)

NZYM Broth (Powder)

I

Immunocytochemistry

Immunofluorescence

Immunohistochemistry

Immunoprecipitation

Immunoprecipitation using Affinity Agarose Resins

L

Laemmli Sample Buffer 2x

M

Modified RIPA Buffer

N

NP-40 Cell Lysis Buffer

Phosphate Buffered Saline (PBS) 1x

Phosphate Buffered Saline (PBS) 10x

Phosphate Buffered Saline Tween-20 1x

R

Red Blood Cell Lysing

Red Blood Cell Lysis Buffer

S

Sodium Citrate Buffer

T

Tissue Homogenization Buffer for ELISA

Tris Glycine Buffer 5x

Tris-Buffered Saline

Tris-Buffered Saline Tween-20

W

Western Blotting Transfer Buffer

Wolfe's Mineral Solution

X

X-Gal Staining Solution

Xylene Cyanol/Bromophenol Blue DNA Loading Buffer

Z

P

PCR, MIQE Guidelines

R

A

Alkaline Lysis Buffers A, B, C

C

Cell Culture Protocols

Cell Staining Buffer

Cell Stimulation Cocktail

Culture Conditions and Types of Growth Media for Mammalian Cells

V

B

Bouin's Fixative

E

Ehrlich's Solution

F

G

Gentian Violet Stain

H

Hollande's Fixative

I

Intracellular Antigen Staining

K

Kovacs' Reagent

P

Papanicolaou's Stain

Paraformaldehyde 2%

S

Schiff's Reagent

T

Tissue Fixative Solution

Tissue Lysates Preparation

Z

Zenker's Fixative

Media Formulations

A

A Broth (Powder)

Amies Broth with Charcoal (Amies Transport Medium) (Powder)

Antibiotic Medium #1 (Powder)

Antibiotic Medium #3 (Powder)

Antibiotic Medium #4 (Powder)

Antibiotic Medium #9 (Powder)

Antibiotic Medium #10 (Powder)

Antibiotic Medium #11 (Powder)

APT (All Purpose Tween) Agar (Powder)

Azide Dextrose Broth (Powder)

B

Bacillus Cereus Medium (BCM) (Powder)

Baird Parker Agar (Powder)

BiGGY Agar (Powder)

Bile Esculin Agar (Powder)

Bile Esculin Azide Agar (Powder)

Bismuth Sulfite Agar (Powder)

Blood Agar Base No. 2 (Powder)

Blood Agar Base, Low pH (Powder)

Blood Agar Base, pH 7.4 (Powder)

Brain Heart Infusion Agar (Powder)

Brain Heart Infusion Broth (Powder)

Brain Heart Infusion Broth w/o Dextrose (Powder)

Brilliant Green Agar (Powder)

Brilliant Green Agar w/Sulfadiazine (Powder)

Brilliant Green Bile Broth 2% (Powder)

Bromthymol Blue

Brucella Agar (Powder)

Brucella Broth (Powder)

Buffered Peptone Water (Powder)

C

Campy Selective Agar Base (Powder)

Casman Medium Base (Powder)

Cetrimide Agar, Non-USP (Pseudosel Agar®) (Powder)

Chapman Stone Medium (Powder)

CLED Agar (Powder)

CLED Agar, Bevis (Powder)

Clostridium Difficile Agar (Powder)

Clostrisel Agar (Powder)

Columbia Blood Agar Base (Powder)

Columbia CNA Agar (Powder)

Cooked Meat Medium (Powder)

Corn Meal Agar (Powder)

D

Decarboxylase Broth Moeller (Powder)

D/E Neutralizing Agar (Powder)

Deoxycholate Agar (Powder)

Deoxycholate Citrate Agar (Powder)

Deoxycholate Citrate Lactose Sucrose (DCLS) Agar (Powder)

Deoxycholate Lactose Agar (Powder)

Dermatophyte Test Medium (DTM Test Agar) (Powder)

Dextrose Agar (Powder)

Dextrose Broth (Powder)

Dextrose Phosphate Broth (Powder)

Dextrose Starch Agar (Powder)

Dextrose Tryptone Agar (Powder)

Dextrose Tryptone Broth (Powder)

Deoxyribonuclease (DNase) Test Agar (Powder)

Deoxyribonuclease (DNase) Test Agar w/Toludine Blue (Powder)

E

Earle’s Balanced Salts (Powder)

EC Medium (Powder)

EC Medium w/MUG (Powder)

Elliker Broth (Powder)

Eosin Methylene Blue Agar (Powder)

Eosin Methylene Blue Agar, Levine (Powder)

Eugonic Broth (Powder)

F

Flow Cytometry General Protocol

Formaldehyde Gel Running Buffer

Fluid Thioglycollate Medium w/K Agar (Powder)

G

Glycerol Tolerant Gel Buffer 20X

H

H Broth (Powder)

Hanks Balanced Salts (10X) (Powder)

Hollande's Fixative

L

L-Broth (Luria Broth) (Powder)

Lambda Agar (Powder)

Lambda Broth (Powder)

LB Agar Lennox (Powder)

LB Agar Lennox, Animal Free (Powder) (Lennox L agar)

LB Agar Miller (Powder)

LB Agar Miller, Animal Free (Powder) (Miller's LB agar, Luria-Bertani agar)

LB Broth Lennox (Powder)

LB Broth Lennox, Animal Free (Powder) (Lennox L broth base)

LB Broth Miller (Powder)

LB Broth Miller, Animal Free (Powder) (Miller's LB broth, Luria-Bertani broth)

LPGA Agar Medium

M

M63 Medium (Powder)

M9 Minimal Salts (Powder)

N

Nutrient Agar (Powder)

Nutrient Agar 1.5% (Powder)

Nutrient Broth (Powder)

NZ Broth (Powder)

NZC Broth (Powder)

NZCYM Agar (Powder)

NZM Agar (Powder)

NZM Broth (Powder)

NZYDT Broth (Powder)

NZYM Agar (Powder)

NZYM Broth (Powder)

S

SOB Agar (Powder)

SOB Broth (Powder)

SOC Broth (Powder)

Sodium Citrate Buffer

STAB Agar (Powder)

Super Broth (Powder)

Synthetic Amino Acid Medium, Bacteriological, SAAM-B (Powder)

Synthetic Amino Acid Medium, Fungal, SAAM-F (Powder)

Synthetic Sea Water

T

Terrific Broth (Powder)

Terrific Broth, Complete with Carbon Source (Powder)

Terrific Broth, Modified for Genomics (Powder)

Terrific Broth, Modified for Fermentation, non-animal (Powder)

Thermophilus Vitamin-Mineral Stock 100X (TYE Media, Castenholz Media) (Powder)

Thermophilus Vitamin-Mineral Stock 1000X (TYE Media, Castenholz Media) (Powder)

Tryptone Agar (Powder)

Tryptone Broth (Powder)

Two YT Broth (TY Broth) (Powder)

Y

Yeast Malt Extract Broth

YT Broth (Powder)

Molecular Biology

#

5-Fluoroorotic Acid Monohydrate

A

Acrylamide (30%)

Acrylamide (40%)

B

Bradford's Reagent

Bromthymol Blue

C

Coomassie Blue Solution

D

Denhardt Solution (50x)

Denhardt Solution (100x)

DEPC Treated Water

Destain Solution Water

DNA Loading Buffer (Orange G)

DNA/RNA Extraction Kit (genesig®)

E

ELISA Blocking Solution

ELISA Coating Solution

ELISA Sample Collection and Storage

F

Flow Cytometry General Protocol

Formaldehyde Gel Running Buffer

G

Glycerol Tolerant Gel Buffer 20X

I

Immunocytochemistry

Immunofluorescence

Immunohistochemistry

Immunoprecipitation

Immunoprecipitation using Affinity Agarose Resins

L

Laemmli Sample Buffer 2x

M

Modified RIPA Buffer

N

NP-40 Cell Lysis Buffer

Phosphate Buffered Saline (PBS) 1x

Phosphate Buffered Saline (PBS) 10x

Phosphate Buffered Saline Tween-20 1x

R

Red Blood Cell Lysing

Red Blood Cell Lysis Buffer

S

Sodium Citrate Buffer

T

Tissue Homogenization Buffer for ELISA

Tris Glycine Buffer 5x

Tris-Buffered Saline

Tris-Buffered Saline Tween-20

W

Western Blotting Transfer Buffer

Wolfe's Mineral Solution

X

X-Gal Staining Solution

Xylene Cyanol/Bromophenol Blue DNA Loading Buffer

Z

PCR

P

PCR, MIQE Guidelines

R

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