Alkaline Lysis Buffer A Recipe

1. Add the following to 100ml distilled H₂O
   • 5ml of 20% SDS
   • 2ml of 10M NaOH

Alkaline Lysis Buffer B Recipe

1. Add the following to 100ml distilled H₂O
   • 29.4g KAc
   • 11.5ml of Glacial Acetic Acid

Alkaline Lysis Buffer C Recipe

1. Add the following to 900ml distilled H₂O
   • 9g Glucose
   • 3g Tris
   • 20ml of 0.25M EDTA
2. q.s. to 1000ml with distilled H₂O
3. Autoclave using standard conditions
4. Allow to cool to room temperature
5. Store at 4^oC

When ready to use add 0.02g lysozyme to 100ml of alkaline lysis buffer C to yield alkaline lysis buffer A.