Amies fomulated a medium for transport of microorganisms using a balanced salt solution. Amies Medium with Charcoal is a non-nutritional, phosphate buffered type medium used to maintian the viablilty of microorganisms without a significant increase in growth.
Dissolve 18.05 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Component | g/liter |
|---|---|
| Calcium Chloride | 0.1 |
| Charcoal | 10.0 |
| Magnesium Chloride | 0.1 |
| Potassium Chloride | 0.2 |
| Monopotassium Phosphate | 2.5 |
| Sodium Chloride | 3.0 |
| Disodium Phosphate | 1.15 |
| Sodium Thioglycollate | 1.0 |
| Total: 18.05g/Liter |
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