Dehydrated culture media suitable for the microbial assay and detection of antibiotics in milk and plate counts in pharmaceutical products. The activity of an antibiotic can be demonstrated under suitable conditions by its inhibitory effect on organisms.
Dissolve 50 grams per liter of ddH2O. Heat with stirring until dissolved. Dispense into appropriate containers, loosen caps and autoclave (15psi) for 15 minutes at 121ºC.
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
| Component | g/liter |
|---|---|
| Agar | 20 |
| Dextrose | 2.5 |
| Potassium Phosphate, Dibasic | 2.5 |
| Sodium Chloride | 5 |
| Papaic Digest of Soybean Meal | 3 |
| Pancreatic Digest of Casein | 17 |
| Total: 50g/Liter |
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