Medium for culturing Lactobacilli from meat products. A non-selective medium supporting growth of many other bacteria.
Dissolve 58.43grams per liter of distilled/deionized water, heating to boiling with stirring until completely solubilized.
Dispense into appropriate containers, loosen caps and autoclave for 15-30 minutes at 121ºC (15 psi).
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Component | g/liter |
|---|---|
| Yeast Extract . | 7.5 |
| Dextrose | 10.0 |
| Casein Digest Peptone. | 10.0 |
| Sodium Citrate | 5.0 |
| Potassium Phosphate | 5.0 |
| Sodium Chloride | 5.0 |
| Tween 80 | 0.2 |
| Sodium Carbonate | 1.25 |
| Magnesium Sulfate | 0.8 |
| Magnesium Chloride | 0.14 |
| Ferrous Sulfate | 0.04 |
| Agar | 13.5 |
| Total: 58.43g/Liter |
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