Selective isolation of coagulase positive Staphylococci in food.
Dissolve 60grams per liter of ddH2O, heating to boiling with stirring until completely solubilized. Dispense into appropriate containers, loosen caps and autoclave for 15-30 minutes at 121ºC (15 psi). After cooling to 45-50°C add 10mL of a sterile I% Potassium Tellurite solution and 50mL of sterile egg yolk emulsion.
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
| Component | g/liter |
|---|---|
| Casein Digest Peptone | 10.0 |
| Lithium Chloride | 5.0 |
| Beef Extract | 5.0 |
| Agar | 17.0 |
| Yeast Extract | 1.0 |
| Glycine | 12.0 |
| Sodium Pyruvate | 10.0 |
| Total: 60g/Liter |
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