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Bradford's Reagent Recipe

Caution: Always add acid slowly into water and do not add water into acid.


Recipe

  1. Dissolve 50mg of Coomassie Blue G250 in 50ml of methanol
  2. Add 100ml of 85% H3PO4 to the solution from step 1
  3. Add the solution from step 2 into 500ml of H2O and mix
  4. Filter to remove and precipitates
  5. Add an additional 350ml of H2O
  6. Store at 4oC.
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