Brain Heart Infusion Agar is used for cultivating a wide variety of fastidious and non-fastidious microorganisms, including bacteria, yeasts and fungi
Dissolve 52 grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized, adjusting pH as necessary. If required, add sodium bicarbonate. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Component | g/liter |
|---|---|
| Brain Heart Infusion (from solids) | 8.0 |
| Peptic Digest of Animal Tissue | 5.0 |
| Pancreatic Digest of Casein | 16.0 |
| Sodium Chloride | 5.0 |
| Dextrose | 2.0 |
| Disodium Phosphate | 2.5 |
| Agar | 13.5 |
| Total: 52g/Liter |
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