Brain Heart Infusion Broth is used for the cultivation fastidious microorganisms, including streptococci, pneumococci and meningococci from a variety of clinical and non-clinical materials.
Dissolve 37g per liter of ddH2O, heating with stirring until completely solubilized. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Component | g/liter |
|---|---|
| Brain Heart Infusion (from solids) | 17.5 |
| Pancreatic Digest of Gelatin | 10.0 |
| Sodium Chloride | 5.0 |
| Dextrose | 2.0 |
| Disodium Phosphate | 2.5 |
| Total: 37g/Liter |
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