Brilliant Green Agar was first described by Kristensen et al. in 1925 and later modified by Kauffman in 1933. It is classified by the USP as a highly selective medium for the recovery of Salmonella species except for tyhphoid and paratyphoid strains. Brilliant Green dye inhibits Gram positive and most Gram negative bacteria. Phenol red serves as a pH indicator for the fermentation of lactose or sucrose in the medium. Osborne and Stokes used 0.1% Sodium Sulfapyridine to enhance the recovery of Salmonella from whole egg and egg yolk. The use of sulfonamides further inhibits Escherichia coli and Proteus sp.
Dissolve 58grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized.
Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Component | g/liter |
|---|---|
| Agar | 20.0 |
| Lactose | 10.0 |
| Sucrose | 10.0 |
| Pancreatic Digest of Casein | 5.0 |
| Peptic Digest of Animal Tissue | 5.0 |
| Sodium Chloride | 5.0 |
| Yeast Extract | 3.0 |
| Phenol Red | 0.08 |
| Sulfadiazine | 0.08 |
| Brilliant Green | 0.0125 |
| Total: 58.1725g/Liter |
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