Campy Selective Agar Base (Powder) is highly nutritive medium for the selective isolation of Campylobacter jejuni and Campylobacter coli.
Dissolve 37 grams per liter of distilled/deionized (DDI) water. Heat with stirring. Boil 1 minute until completely solubilized. Adjust pH as necessary. Dispense into appropriate containers. Loosen caps or cover with foil. Autoclave for 15 minutes at 121ºC (15psi). Aseptically add 5% lysed horse blood and 10ml of filter sterilized aqueous solution of antimicrobials.
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Component | g/liter |
|---|---|
| Enzymatic Digest of Animal Tissue | 10g |
| Enzymatic Digest of Casein | 10g |
| Sodium chloride | 5g |
| Agar | 12g |
| Total: 37g/Liter |
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