The Cell Stimulation and Protein Transport Inhibitor Cocktail is a pre-mixed cocktail used to induce cytokine production and inhibit their intracellular transport for flow cytometry detection. The solution is composed of an optimized mixture of brefeldin A, ionomycin, monesin, and PMA. PMA and ionomycin induce cytokine production in many cell types and brefeldin A and monensin are protein transport inhibitors.