Columbia Blood Agar Base is usually prepared with horse, sheep, or rabbit blood to determine the hemolytic reactions of fastidious pathogenic microorganisms. Columbia Blood Agar Base can be prepared as Blood Agar or Chocolate Agar or used without blood as a general purpose medium. This medium contains a highly nutritious combination of peptones. The addition of starch enhances the recovery of Neisseria, and hemolytic reactions of some streptococci.
Dissolve 43grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized.
Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Prepare 5 to 10% blood agar by adding the appropriate volume of sterile defibrinated blood to melted sterile agar medium, cooled to 45-50°C. Dispense into Petri dishes or tubes.
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Component | g/liter |
|---|---|
| Casein Digest Peptone | 5.0 |
| Corn Starch | 1.0 |
| Peptic Digest of Animal Tissue | 8.0 |
| Sodium Chloride | 5.0 |
| Yeast Enriched Peptone | 10.0 |
| Agar | 14.0 |
| Total: 43g/Liter |
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