A selective medium for isolation of gram positive bacteria. Most often utilized with blood added. Eliner, et al. added colistin and nalidixic acid to Columbia Agar to suppress the growth of Gram negative bacteria. The addition of 5-10% sheep red blood cells to the base demonstrates hemolytic patterns of the Staphylococci and Streptococci.
Dissolve 43 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi). Prepare 5-10% blood agar by adding the appropriate volume of sterile defibrinated blood to melted sterile agar medium, cooled to 45-50°C. Dispense into Petri dishes or tubes
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Component | g/liter |
|---|---|
| Agar | 14.0 |
| Colistin | 0.015 |
| Corn Starch | 1.0 |
| Nalidixic Acid | 0.010 |
| Sodium Chloride | 5.0 |
| Yeast Enriched Peptone | 10.0 |
| Peptic Digest of Animal Tissue | 8.0 |
| Casein Digest Peptone | 5.0 |
| Total: 43g/Liter |
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