Suitable for the cultivation of anaerobes and stock culture maintenance. Used to differentiate saccharolytic from proteolytic Clostridium sp. Saccharolytic species form acid and gas without digesting the meat. Proteolytic species form sulfur compounds (putrid smell) and break down the meat to amino acids (blackening). Also suitable for recovery of Clostridium botulinum from foods. Expected cultural response after 48-72 hours at 35.0°C.
Dissolve 1.25grams per ml of distilled/deionized (DDI) water.
Autoclave for 15 minutes at 121ºC (15psi)
Dehydrated medium may be stored at 2-30°C. After opening container, store recapped at same temperature in low humidity environment. Discard if not free flowing or if the color has changed from the original brown pellets. Prepared media should be kept at 4°C and used within a short period of time.
| Component | g/liter |
|---|---|
| Beef Heart | 454 |
| Dextrose | 2 |
| Proteose Peptone | 20 |
| Sodium Chloride | 5 |
| Total: 481g/Liter |
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