General purpose medium for fungi cultivation.
Corn Meal Agar is valuable for morphologic differentiation of many yeast-like organisms. It suppresses vegetative growth of many fungi while stimulating sporulation. Corn Meal Agar allows Candida albicans to produce chlamydospores which is one of the best criterion for identification. Kelly and Funigello reported that the addition of 1% Tween 80 enhanced chlamydospore formation.
Dissolve 17 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Component | g/liter |
|---|---|
| Corn meal | 15 |
| Agar | 2 |
| Total: 17g/Liter |
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