Deoxycholate Lactose Agar was formulated by Leifson for the detection of coliforms in water, dairy products and other food materials. USBio recommends Violet Red Bile Agar as the medium of choice for isolating coliforms from food products. Growth of coliforms which ferment lactose produce red colonies surrounded by a zone of red precipitate. The fermentation of lactose produces acid products which lowers the pH of the medium and the neutral red dye in the medium turns bright red. Sodium citrate and sodium deoxycholate are added to inhibit the growth of Gram positive bacteria.
Dissolve 42.5grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized. Do not autoclave.
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Component | g/liter |
|---|---|
| Peptic Digest of Animal Tissue | 5.0 |
| Lactose | 10.0 |
| Casein Digest Peptone | 5.0 |
| Sodium Chloride | 5.0 |
| Sodium Citrate | 2.0 |
| Sodium Deoxycholate | 0.5 |
| Neutral Red | 0.033 |
| Agar | 15.0 |
| Total: 42.5g/Liter |
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