Dermatophyte Test Medium is used for the selective isolation of dermatophyic fungi.
Suspend 40.7 grams of the powder in IL of purified water. Mix thoroughl. Heat wih frequent agitationand boil for 1 minute to dissolve the powder completely. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi). Cool to 50°C. Aseptically add sterile gentamicin (0.1g/L) and sterile chlortetracycline (0.1g/L).
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
| Component | g/liter |
|---|---|
| Agar | 20.0 |
| Cycloheximide | 0.5 |
| Dextrose | 10.0 |
| Phenol Red | 0.2 |
| Soy Peptone | 10.0 |
| Total: 40.7g/Liter | |
| Supplements (Added post autoclaving): | |
| Gentamicin | 0.1 |
| Chlortetracycline | 0.1 |
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