A standard methods medium for dairy products testing. Dextrose Phosphate Broth is a modification of yeast autolysate agar. The addition of dibasic sodium phosphate is used to control hydrogen ion concentration which is produced during the utilization of dextrose. Low pH is inhibitory to Brucella.
Dissolve 32 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. If required, add sodium bicarbonate. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Component | g/liter |
|---|---|
| Casein Digest Peptone | 10.0 |
| Yeast Extract | 2.0 |
| Sodium Chloride | 5.0 |
| Sodium Bisulfite | 0.1 |
| Peptic Digest of Animal Tissue | 10.0 |
| Dextrose | 1.0 |
| Disodum Phosphate | 3.0 |
| Sodium Citrate | 1.0 |
| Total: 32g/Liter |
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