EC Medium was developed by Hajna and Perry to differentiate between fecal and non-fecal coliform bacteria. It is a buffered lactose broth which also contains bile salts which inhibits Gram positive cocci and spore formers. It was designed to improve the isolation and detection of E coli in contaminated water, milk and shellfish. APHA recommends that EC Broth be used for the determination of water pollution. Coliforms of fecal origin are detected by growth at 44.5°C. Fermentation of lactose is observed by gas bubbles present in an inverted fermentation tube.
Dissolve 37grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized.
Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi)
Store powdered media at 4°C Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should stored at 4°C and used within a short period of time.
| Component | g/liter |
|---|---|
| Casein Digest Peptone | 15.0 |
| Deoxyribonucleic Acid | 2.0 |
| Papaic Digest of Soybeans | 5.0 |
| Sodium Chloride | 5.0 |
| Agar | 15.0 |
| Toluidine Blue O | 0.1 |
| Total: 37g/Liter |
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