Selective medium used to detect gram negative enteric bacteria. Contains double the conc. of lactose than EMB (HH&T) and no sucrose.
Dissolve 37.5grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized.
Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi)
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Component | g/liter |
|---|---|
| Pancreatic Digest of Gelatin | 10.0 |
| Lactose | 10.0 |
| Dipotassium Phosphate | 2.0 |
| Methylene Blue | 0.065 |
| Eosin Y | 0.4 |
| Agar | 15.0 |
| Total: 37.5g/Liter |
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