A sterility testing medium where clarity of the medium is required, Fluid Thioglycollate Medium w/K Agar is suitable for isolation of A. acidoterrestris spores from fruit juices. K Agar is a native form that exhibits exceptional clarity in gel form, allowing for easier visual detection of bacterial growth. Dextrose, casitone, cystine, and yeast extract provide the growth factors necessary for bacterial replication. Sodium provides essential ions. The reducing agents sodium thioglycollate and cystine lower the redox potential of the medium, thereby supporting anaerobiosis. Sodium thioglycollate also prevents accumulation of peroxides which are lethal to some microorganisms. Resazurin serves as redox indicator.
Dissolve 29.45g in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Add additional water to bring the solution to1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Note: After storage of the medium, if 30% of the medium demonstrates oxidation (color change of indicator), reheat the medium once in boiling water or steam bath.
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
| Component | g/liter |
|---|---|
| L-Cystine | 0.5 |
| Dextrose | 5.0 |
| K Agar | 0.45 |
| Potassium Chloride | 2.5 |
| Resazurin | 0.001 |
| Sodium Chloride | 0.5 |
| Sodium Thioglycollate | 0.5 |
| Casitone | 15.0 |
| Yeast Extract | 5.0 |
| Total: 29.451g/Liter |
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