Recipe
Stock Solution
- Dissolve 3.8g of Giemsa powder into 250ml of methanol
- Heat the solution from step 1 to ~60oC
- Slowly add in 250ml of glycerin to the solution from step 2
- Filter the solution from step 3
- The solution needs to stand a period of time prior to use. Although times vary based on who you ask a minimum of two months is usually recomended
Working Solution
- Add 10ml of stock solution to 80ml of distilled water and 10ml of methanol