Buffered salt formulation used as a base to prepare minimal media for propagation of E. coli and plasmid amplification. A complete media that requires one or more additional salts and carbon source.
Dissolve 15.6 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
| Component | g/liter |
|---|---|
| Ammonium Sulfate | 2 |
| Potassium Phosphate mono | 13.6 |
| Ferrous Sulfate | 0.0005 |
| Total: 15.6g/Liter | |
| Not included: Carbohydrate Solution & Magnesium Sulfate Solution (MgSO4•7H2) | |
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