Recipe
- 10 mM Tris, pH 7.4
- 100 mM NaCl
- 1 mM EDTA
- 1 mM EGTA
- 1 mM NaF
- 20 mM Na4P2O7
- 2 mM Na3VO4
- 1% Triton X-100
- 10% glycerol
- 0.1% SDS
- 0.5% deoxycholate
- plus protease inhibitors: Aprotinin, leupeptin, pepstatin: 1ug/ml each
- Add 1mM PMSF immediately before use.
