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NP-40 Cell Lysis Buffer


Recipe

  • 50mM Tris-HCl pH 8.0
  • 150mM NaCl
  • 1% NP-40
  • plus protease inhibitors: Aprotinin, leupeptin, pepstatin: 1ug/ml each
  • Add 1mM PMSF immediately before use.
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