Slightly alkaline enrichment media for cultivating fastidious organisms.
Nutrient Agar 1.5% is a basic media composed of a simple peptone and a beef extract. The gelatin peptone contributes organic nitrogen in the form of amino acids and long-chain fatty acids. The beef extract provides additional vitamins, carbohydrates, salts and other organic nitrogen compounds. If needed other enrichments may be added to the prepared media or during the preparation of the dehydrated powder. Blood may be prepared with Nutrient Agar 1.5%
Dissolve 31.0 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. If required, add sodium bicarbonate. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
| Component | g/liter |
|---|---|
| Agar | 15 |
| Beef Extract | 3 |
| Peptone | 5 |
| Sodium Chloride | 8 |
| Total: 31g/Liter |
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