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SOB Agar (Powder)

Catalog No: S1015

Media for propagation E. coli host cells.

Directions per Liter:

Dissolve 45.5 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. If required, add sodium bicarbonate. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).

Storage and Stability:

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Components
Componentg/liter
Tryptone20
Yeast Extract5
Sodium Chloride0.5
MgSO4•7H2O5
Agar15
Total: 45.50g/Liter
500g makes 11 liters
  • BSE-Free: All raw materials of animal origin are sourced from USDA-designated BSE-Free countries.
  • Dust-Free: Specially milled powder blends are designed to eliminate or greatly reduce airborne particulates.
  • Homogeneous: Proprietary blending technology ensures complete mixing of components.
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