Media base for propagation E.coli host cells.
Suspend 30.5g per liter of ddH2O. Mix thoroughly. Adjust to desired pH. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Component | g/liter |
|---|---|
| MgSO4•7H2O | 5 |
| Sodium Chloride | 0.5 |
| Tryptone | 20 |
| Yeast Extract B | 5 |
| Total: 30.5g/Liter | |
| 500g makes 16.4 liters |
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