Rich media base for propagation E.coli host cells.
Dissolve 57 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Component | g/liter |
|---|---|
| Sodium Chloride | 5 |
| Tryptone | 32 |
| Yeast Extract | 20 |
| Total: 57g/Liter | |
| 500g makes 8.8 liters |
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