Terrific Broth is a highly enriched bacteriological medium specifically designed by Tartof and Hobbs to increase the yield of plasmid DNA derived from suspension cultures of E. coli due in part to the increased buffering capacity derived from using mono and dibasic phosphates.
Dissolve 47.6g grams in 800-900ml of ddH2O stirring gently until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Dispense into appropriate containers, loosen caps and autoclave (15psi) for 15 minutes at 121ºC.
The original formulation calls for the addition of 4ml glycerol per liter broth solution.
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Component | g/liter |
|---|---|
| Potassium phosphate dibasic | 9.4 |
| Potassium phosphate monobasic | 2.2 |
| Tryptone | 12 |
| Yeast Extract | 24 |
| Total: 47.6g/Liter | |
| Add 4ml glycerol/liter final solution. 500g makes 10.5 liters |
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