Terrific Broth is a highly enriched bacteriological medium specifically designed by Tartof and Hobbs to increase the yield of plasmid DNA derived from suspension cultures of E. coli due in part to the increased buffering capacity derived from using mono and dibasic phosphates.
Non-animal sourced Tryptone: Utilizes a plant-based tryptone manufactured by a controlled enzymatic hydrolysis of plant proteins containing a mixture of peptides and free amino acids which provide a source of organic nitrogen and growth factors.
Dissolve 47.6 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. If required, add sodium bicarbonate. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
| Component | g/liter |
|---|---|
| Potassium phosphate dibasic | 9.4 |
| Potassium phosphate monobasic | 2.2 |
| Tryptone (Animal Protein Free) | 12 |
| Yeast Extract | 24 |
| Total: 47.6g/Liter | |
| 500g makes 10.5 liters |
We accept the following payment methods as well as pay-by-invoice.
