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Tissue Homogenization Buffer for ELISA


Recipe

Stock Solution:
  1. Weigh out 0.025 grams of Aprotinin and Luepeptin and dissolve in 25ml of water
  2. Mix and aliquot into 70ul single use aliquots
To prepare homogenization buffer:
  1. Add 50ul of stock solution to 100ml of PBS
  2. Sterilze by filtration

Final concentration is 0.5ug/ml

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