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Wolfe's Mineral Solution

Recipe

  1. Add 1.5g nitrilotriacetic acid to approximately 500 ml of water and adjust to pH 6.5 with KOH to dissolve.
  2. Add the following:
    • 3.0g MgSO4-7H2O
    • 0.5g MnSO4-H2
    • 1.0g NaCl
    • 0.1g FeSO4-7H2O
    • 0.1g CoCl2-6H2O
    • 0.1g CaCl2
    • 0.1g ZnSO4-7H2O
    • 0.01g CuSO4-5H2O
    • 0.01g AlK(SO)4-12H2O
    • 0.01g H3BO3
    • 0.01g Na2MoO4-2H2O
  3. q.s. to 1000ml
  4. Filter sterilize
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