Anti -4-1BB Ligand (TNFSF9, CD137, CDw137, ILA, MGC2172, T-cell antigen 4-1BB homolog,
T-cell antigen ILA, Tumor necrosis factor receptor superfamily member 9 precursor) (PE)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Purified |
FC |
|
| 4-1BB ligand (4-1BBL; also CD137L) is a 32kD type II transmembrane protein that belongs to TNF superfamily (TNFSF) of molecules. 1-4 The human 4-1BBL cDNA encodes a 254aa protein that contains a 25aa N-terminal cytoplasmic domain, a 23aa transmembrane segment, and a 206aa C-terminal extracellular region. 5 The extracellular domain (ECD) of 4-1BBL has a jelly-roll, beta-sandwich tertiary structure that is similar to other TNFSF members. There is only one cysteine in the human ECD, and no potential N-linked glycosylation sites. The potential exists, however, for O-linked glycosylation. The human 4-1BBL ECD shares 32% and 35% aa identity with mouse and rat ECD, respectively. In the cytoplasmic domain, human 4-1BBL | | | is 55aa shorter than the equivalent region in rodents. 4-1BBL is expressed by activated B cells, macrophages, dendritic cells, activated T cells, neurons, and astrocytes. 2, 3, 6 A 26kD soluble form of 4-1BBL is known to occur in human. Although it is presumably generated by MMP activity, its amino acid size is currently unreported. 4 The soluble form is bioactive. Human | | | 4-1BBL signals through both CD137/4-1BB and itself. Its cytoplasmic tail participates in reverse signaling that induces apoptosis in T cells and cytokkine secretion (IL-6; TNF-alpha) by monocytes. 7, 8 4-1BBL binding to CD137/4-1BB produces a number of effects. | | | It seems to play a key role in the T cell recall response. It maintains T cell numbers at the end of a primary response, and induces CD4 + and CD8 + T cells to proliferate and secrete cytokines such as IL-2 and IFN- in CD4 + cells, and IFN- in CD84-1BB Ligand/TNFSF9. | | | Catalog # | 0004-01M | | Applications | Suitable for use in Flow Cytometry. Other applications not tested. | | Optimal dilutions to be determined by the researcher. | | Recommended Dilution | Flow Cytometry: Designed to quantitatively determine the percentage of cells bearing 4-1BB Ligand/TNFSF9 within a population and qualitatively determine the density of 4-1BB Ligand/TNFSF9 on cell surfaces by flow cytometry. Washed cells are incubated with the phycoerythrin-labeled monoclonal antibody, which binds to cells expressing 4-1BB Ligand/TNFSF9. Unbound phycoerythrin-conjugated antibody is then washed from the cells. Cells expressing 4-1BB Ligand/TNFSF9 are fluorescently stained, with the intensity of staining directly proportional to the density of expression of 4-1BB Ligand/TNFSF9. Cell surface expression of 4-1BB Ligand/TNFSF9 is determined by flow cytometric analysis using 488nm wavelength laser excitation and monitoring emitted fluorescence with a detector optimized to | | collect peak emissions at 565-605nm. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Clone Type | Monoclonal | | Isotype | IgG2b | | Clone No | 9H4 | | Host | Mouse | | Source | Human | | Concentration | ~50ug/ml | | Form | Supplied as a liquid in 50ug antibody in 1ml PBS, 0.1% sodium azide. | | Purity | Purified | | Immunogen | Synthetic peptide corresponding to human 4-1BB Ligand /TNFSF9- Phycoerythrin. | | Specificity | Recognizes human 4-1BB Ligand /TNFSF9- Phycoerythrin. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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