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0014-20H Goat Anti-14-3-3, tau (14-3-3 Protein theta, 14-3-3 Protein tau, 14-3-3 Protein T-cell, Protein HS1, YWHAQ)

Specifications
References
Clone Type
Polyclonal
Host
Goat
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_006817.1
Gene ID
10971
Shipping Temp
Blue Ice
Storage Temp
-20°C

The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms that have been identified in mammals. The 14-3-3 tau, a subtype of the 14-3-3 family of proteins, was found in T Cells, brain and testes. This 14-3-3 tau is upregulated in patients with amyotrophic lateral sclerosis. Recombinant human YWHAQ was expressed in E. coli and purified by using conventional chromatography techniques.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:4000 Western Blot: 0.03-0.1ug/ml, observed in human brain (hippocampus) lysates on ~28kD bands Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-DDRKQTIDNSQ, from human YWHAQ, at the internal region of the protein (NP_006817.1). Species sequence homology: Canine, mouse and rat.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human YWHAQ.
References
1. Howlett KF, Sakamoto K, Garnham A, Cameron-Smith D, Hargreaves M. Resistance exercise and insulin regulate AS160 and interaction with 14-3-3 in human skeletal muscle. Diabetes. 2007 Jun;56(6):1608-14. Epub 2007 Mar 16.
USBio References
No references available
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