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You are here:Home » Antibodies » Abs to 14-3-3 » Anti -14-3-3, tau (14-3-3 Protein theta, 14-3-3 Protein tau, 14-3-3 Protein T-cell, Protein HS1, YWHAQ)

Anti -14-3-3, tau (14-3-3 Protein theta, 14-3-3 Protein tau, 14-3-3 Protein T-cell,
Protein HS1, YWHAQ)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms that have been identified in mammals. The 14-3-3 tau, a subtype of the 14-3-3 family of proteins, was found in T Cells, brain and testes. This 14-3-3 tau is upregulated in patients with amyotrophic lateral sclerosis. Recombinant human YWHAQ was expressed in E. coli and purified by using conventional chromatography techniques.
Catalog #0014-20H
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:4000
Western Blot: 0.03-0.1ug/ml, observed in human brain (hippocampus) lysates on ~28kD bands
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to C-DDRKQTIDNSQ, from human YWHAQ, at the internal region of the protein (NP_006817.1).
SpecificityRecognizes human YWHAQ. Species sequence homology: Canine, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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