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029950 Mouse Anti-ALPL (Alkaline phosphatase liver/bone/kidney)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P05186
Isotype
IgG1
Clone Number
13B28
Grade
Purified
Applications
E FC IHC WB
Crossreactivity
Hu
Gene ID
249
Shipping Temp
Blue Ice
Storage Temp
-20°C
HOPS; TNAP; APTNAP; TNSALP; AP-TNAP

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants have been described.

Applications
Western Bloting: 1:500-1:2000. Immunohistochemistry: 1:200-1:1000. Flow cytometry: 1:200-1:400. ELISA: Suggested dilution 1:10000. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified recombinant fragment of human ALPL expressed in E. coli.
Form
Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer.
Purity
Purified.
Specificity
Recognizes human ALPL.
References
1. J Rheumatol. 2009 Dec;36(12):2758-65., 2. Calcif Tissue Int. 2009 Sep;85(3):228-34.
USBio References
No references available
Conjugates
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