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031078 Mouse Anti-MAP1LC3A (LC3, APG8, Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, Microtubule-associated protein 1 light chain 3 alpha)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
Q9H492
Isotype
IgG1,k
Clone Number
166AT1234
Grade
Affinity Purified
Applications
IC IF IHC WB
Crossreactivity
Hu Mo Rt
Gene ID
84557
Gene #
MAP1LC3A
Shipping Temp
Blue Ice
Storage Temp
-20°C
MAP1LC3A, Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, Microtubule-associated protein 1 light chain 3 alpha

MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene.

Applications
Suitable for use in Immunocytochemistry, Immunochistochemistry, Immunofluorescence amd Western Blot. Other applications not tested.
Recommended Dilution
Immunocytochemistry/Immunofluorescence: 1:25-1:200 Immunohistochemistry: 1:50 -1:100 Western Blot: 1:1,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Full length recombinant human LC3 (APG8) protein.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human LC3. Species Crossreactivity: mouse and rat
References
Autophagy negatively regulates Wnt signalling by promoting Dishevelled degradation. Gao C, et al. Nat Cell Biol, 2010 Aug. PMID 20639871. The prolyl isomerase Pin1 induces LC-3 expression and mediates tamoxifen resistance in breast cancer. Namgoong GM, et al. J Biol Chem, 2010 Jul 30. PMID 20479004. Protein kinase C inhibits autophagy and phosphorylates LC3. Jiang H, et al. Biochem Biophys Res Commun, 2010 May 14. PMID 20398630. Processing of autophagic protein LC3 by the 20S proteasome. Gao Z, et al. Autophagy, 2010 Jan. PMID 20061800. Defective autophagy associated with LC3 puncta in epothilone-resistant cancer cells. Shen S, et al. Cell Cycle, 2010 Jan 15. PMID 20023420.
USBio References
No references available
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