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031143 Mouse Anti-Myc-Tag (c-myc, myc-1 epitope tag)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Swiss Prot
P01106
Isotype
IgG1
Clone Number
9.00E+10
Grade
Affinity Purified
Applications
E WB
Shipping Temp
Blue Ice
Storage Temp
-20°C
Tag from c-Myc protein

Epitope tags consisting of short sequences recognized by well-characterizated monoclonal antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYA) and Myc Tag (AEEQKLISEEDLLRKRREQLKHKLE), recognized by monoclonal antibody clones 12CA5 and 9E10, respectively, are illustrative examples. Our anti-Myc monoclonal antibody (Clone 9E10) provides a simple solution to detect the expression of a Myc-tagged protein in cells.

Applications
Suitable for use in Western Blot and ELISA. Other applications not tested.
Recommended Dilutions
Western Blot: 1:2000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH conjugated synthetic peptide encoding c-Myc tag, AEEQKLISEEDLLRKRREQLKHKLE
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes Myc-Tag
References
1. Kolodziej, PA and Young RA. (1991) Methods Enzymol., 194:508-19. 2. Sells MA and Chernoff J. (1995) Gene, 152:187-9.
USBio References
No references available
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