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031430 Mouse Anti-Synuclein, alpha (Alpha-synuclein, SNCA, Non-A beta Component of AD Amyloid, Non-A4 Component of Amyloid Precursor, NACP, PARK1)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P37840
Isotype
IgG1
Clone Number
2A7
Grade
Affinity Purified
Applications
IC IF IHC WB
Crossreactivity
Bo Hu Mo Po Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

Alpha-synuclein is a member of the synuclein protein family, the other two members being β and γ-synuclein, each protein is coded for by a distinct but related gene. α-synuclein was originally isolated as a major synaptic vesicle associated protein from the electric organ of the fish Torpedo (1), and direct homologues of α-synuclein are found in all vertebrates. Later work connected α-synuclein expression with several human brain pathologies, it is a major component of the Lewy bodies of Parkinson’s disease (2). Point mutations of α-synuclein proved to be causative of some forms of familial Parkinson’s disease (3-5). One genetic cause of early onset Parkinson’s disease is duplication or triplication of one of the α-synuclein genes leading to excess production of the protein (6,7). α-synuclein is also found in the Lewy bodies of patients with diffuse Lewy body disease and inclusions in glial cells in the brains of patients with multiple system atrophy and amyotrophic lateral sclerosis. α-synuclein is normally heavily expressed in brain and appears to be localized primarily to presynaptic regions, though not with a typical synaptic vesicle distribution pattern.

031430 has also been used as a capture reagent capable of detecting endogenous α-synuclein in human plasma (8).
Applications
Suitable for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Immunofluorescence (IC): 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Full length recombinant protein corresponding to human SNCA, expressed in E. coli.
Form
Supplied as a liquid in PBS, 5mM sodium azide, 50% glycerol
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human SNCA. No cross-reactivity with either beta or gamma-synuclein. The epitope is in the region 61-95 which corresponds to the "Non-Amyloid beta Component of Alzheimer's disease amyloid" (NAC). Will also bind human alpha-synuclein containing the A30P and A53T Parkinson’s associated mutations. Species Crossreactivity: rat, mouse, bovine and porcine.
References
1. Maroteaux L, Campanelli JT, Scheller RH. Synuclein: a neuron-specific protein localized to the nucleus and presynaptic nerve terminal. J. Neurosci. 8:2804-15 (1988).|2. Lavedan C. The Synuclein Family. Genome Research 8:871-80 (1998).|3. Polymeropoulos, MH et al. Mutation in the alpha-synuclein gene identified in families with Parkinson’s disease. Science 276:2045-7 (1997).|4. Kruger, R et al. Ala30-to-Pro mutation in the gene encoding alpha-synuclein in Parkinson’s disease. Nature Genet. 18:106-8 (1998).|5. Chartier-Harlin, M-C. et al. Alpha-synuclein locus duplication as a cause of familial Parkinson’s disease. Lancet 364:1167-9 (2004).|6. Singleton, AB.et al. Alpha-synuclein locus triplication causes Parkinson’s disease. Science 302:841 (2003).|7. Ibanez, P. et al. Causal relation between alpha-synuclein gene duplication and familial Parkinson’s disease. Lancet 364:1169-71 (2004).|8. Tinsley RD, et al. Sensitive and specific detection of α-synuclein in human plasma. J. Neurosci. Res. 88:2693-700 (2010).
USBio References
No references available
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