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You are here:Home » Growth Factors, Cytokines » Growth Factors-BAFF » BAFF, Soluble (60-mer), Recombinant, Human(highly active) (BLyS, TALL-1, CD257, B Cell Activating Factor, TNFSF13B, Tumor Necrosis Factor Ligand Superfamily Member 13B)

BAFF, Soluble (60-mer), Recombinant, Human(highly active) (BLyS, TALL-1, CD257, B Cell
Activating Factor, TNFSF13B, Tumor Necrosis Factor Ligand Superfamily Member 13B)

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Specifications

BAFF is mainly produced by innate immune cells such as neutrophils, monocytes, macrophages, dendritic cells, follicular dendritic cells. T cells, activated B cells, some malignant B cells and also non-lymphoid cells like astrocytes, synoviocytes and epithelial cells can also produce BAFF. BAFF binds three distinct receptors (BAFF-R, TACI and BCMA) expressed predominantly on B cells, although activated T cells also express BAFF-R. BAFF is a master regulator of peripheral B cell survival, and together with IL-6, promotes Ig class-switching and plasma cell differentiation. Besides its major role in B cell biology, BAFF co-stimulates activated T cells. Deregulated expression of BAFF leads to autoimmune disorders in mice. In humans, elevated levels of soluble BAFF have been detected in the serum of patients with various autoimmune diseases such as Sjoegren syndrome, Rheumatoid arthritis (RA), Multiple sclerosis (MS) and Systemic Lupus Erythematosus (SLE). BAFF has also increased levels in some lymphoid cancers. Processed human BAFF can either remain as a trimer, which is usual for TNF family ligands or assemble into 60-mer composed of 20 trimers. Mouse BAFF 60-mer has been identified in the serum of BAFF transgenic mice. Oligomerization of BAFF 3-mer into 60-mer in human BAFF is prevented by mutation of His218, a residue critical for 3-mer-to-3-mer interactions, but not for receptor binding. Despite the predominant functional role of processed BAFF in vivo, membrane-bound BAFF might also play a role. Indeed, soluble BAFF (3-mer) can trigger BAFF-R but not TACI or BCMA, whereas oligomeric forms of BAFF (BAFF 60-mer), which mimic membrane-bound BAFF, activate all BAFF receptors.
Catalog #044589
Biological ActivityIncreases B cell survival /Proliferation. Increases CD21 / CD23 expression on B cells in vivo. Activates BAFF-R, TACI and BCMA receptors. Works at concentrations <20ng/ml.
SequenceThe extracellular domain of human BAFF (aa 134-285) is fused at the N-terminus to a His-tag.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~19kD reduced ~1100kD native
SourceE. coli
Purity>95% (SDS-PAGE) Endotoxin: <0.01EU/ug protein
Concentration~0.1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from a 0.2um filtered solution in PBS pH 7.2.
SpecificityBinds to human and mouse BAFF-R, TACI and BCMA. Relative binding affinity (EC50) for BAFF-R (human): ~3ng/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesBLyS, TALL-1, CD257, B Cell Activating Factor, TNFSF13B, Tumor Necrosis Factor Ligand Superfamily Member 13B


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