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You are here:Home » Molecular Biology » MB-Growth Factors-EGF » Betacellulin, Recombinant, Human (Probetacellulin, BTC)

Betacellulin, Recombinant, Human (Probetacellulin, BTC)


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Betacellulin (BTC), a member of the EGF family of ligands, is a widely expressed growth factor that was originally identified in conditioned medium of a mouse pancreatic beta cell line (1). BTC is synthesized as a 178 amino acid transmembrane precursor protein that is proteolytically cleaved into an 80 amino acid mature protein (1). Human BTC shares 74% amino acid sequence identity with mouse BTC (1). BTC can bind to and activate the ErbB family of receptor tyrosine kinases, EGFR/ErbB1, HER2/ErbB2, HER3/ErbB3, and HER4/ErbB4 (1,2). BTC can also bind to and activate EGFR/ErbB1 and HER4/ErbB4 homodimers (1). Ligand-receptor interactions result in the activation of the PI3K and Erk pathways (1). BTC induces the proliferation of pancreatic beta cell lines in vitro (2). Moreover, research studies have shown that BTC induces beta cell regeneration and increased insulin secretion in rodent models of diabetes (2-4).
Catalog #137412
SourceRecombinant protein corresponding to aa31-111 from human Betacellulin expressed in E. coli.
Molecular Weight~8.984kD
Biological ActivityThe proliferation of MCF 10A cells treated with increasing concentrations of hBTC was assessed. After treatment, cells were labeled with BrdU, determined by ELISA.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity~98% (SDS-PAGE)
Concentration~0.1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder in PBS, pH 7.2, 20ug BSA. Reconstitute in sterile PBS.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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