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143721 B7-1/CD80 BioAssay™ ELISA Kit, Mouse

Specifications
References
Brand
BioAssay™
Gene ID
12519
Kit Type
Sandwich ELISA
Tests
96
Sensitivity
< 10 pg/ml
Detection Method
Colorimetric
Detection Range
62.5pg/ml-4000pg/ml
Sample Matrix
cell culture supernates and serum
EU Commodity Code
38220000
UN DOT Shipping
UN2796 PGII
Shipping Temp
Blue Ice
Storage Temp
4°C/-20°C

Sandwich ELISA quantitative detection kit for Mouse B7-1/CD80 in cell culture supernates and serum.

The protein CD80 (Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7.1. The cDNA for B7-1 predicts a type I membrane protein, i.e., one synthesized with a signal peptide that is cleaved upon translocation across the endoplasmic membrane. The protein is predicted to contain 2 extracellular domains structurally similar to those of Ig, a hydrophobic transmembrane region, and a short cytoplasmic domain[1].The CD80 and CD86 (601020) genes encode B7-1 and B7-2, respectively, which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types.[2] stated that B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4. The standard product used in this kit is recombinant B7-1,D37—K245,which is composed of two single chains acids with the dipolymer.
Assay Range
62.5pg/ml-4000pg/ml
Sensitivity
< 10 pg/ml
Crossreactivity
There is no detectable cross-reactivity with other relevant proteins.
Uniprot ID
Q00609
Entrez Gene ID
12519
Kit Components
96-well plate pre-coated with anti-B7-1/CD80, Mouse Lyophilized recombinant B7-1/CD80, Mouse standard, 10ng/tube, 2Ea USB Cat No Kit Component 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution) 143697 Sample diluent buffer, 30ml 143698 Antibody diluent buffer, 12ml 143699 ABC diluent buffer, 12ml 143700 TMB color developing agent, 10ml 143701 TMB stop solution, 10ml
Storage and Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.
References
[1]. Reeves, R. H., Patch, D., Sharpe, A. H., Borriello, F., Freeman, G. J., Edelhoff, S., Disteche, C. The costimulatory genes Cd80 and Cd86 are linked on mouse chromosome 16 and human chromosome 3. Mammalian Genome 8: 581-582, 1997.
USBio References
No references available
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