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168770-AF Mouse Anti-Erythrocyte Specific (RBC, Red blood cells, Erythroid Marker, Erythrocyte specific protein) (BSA & Azide Free)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG2b,k
Clone Number
SFL23.6
Grade
Affinity Purified
Applications
FC IF
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
BSA Free
(RBC, Red blood cells, Erythroid Marker, Erythrocyte specific protein)

Mouse Anti-Human Erythrocytes Clone SFL23.6 MAb is directed against an erythroid cell surface antigen. It shows a well defined reactivity with cells of the erythroid lineage at all stages of maturation in the peripheral blood, bone marrow, and fetal liver. Non-erythroid lineages are negative by flow cytometry. Although the exact identity of the antigen has yet to be determined, it has been shown to be distinct from Glycophorin A. This MAb is useful in the diagnosis of erythroleukemia, identification of bone marrow erythroid precursors, gating erythroid nucleated precursor cells from malignant cells in bone marrow specimens.

Applications:  Suitable for use in Immunofluorescence and Flow Cytometry. Other applications not tested.
Recommended Dilution
Flow Cytometry: 0.5-1ug/million cells  Immunofluorescence: 0.5-1ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
RBC. Placenta
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Hepatocytes from 20-22 week-old human fetus. Cellular Localization: Cytoplasmic
Form
Supplied as a liquid in 10mM PBS. Also available with BSA and azide, see 168770.
Purity
Purified by Protein A/G affinity chromatography.
Specificity
Recognizes human Erythrocyte Specific
References
1. Das Gupta, A., Samoszuk, MKI, Papayannopoulou, T, and Stamatoyannopoulos, G: SFL 23.6: A monoclonal antibody reactive with CFU-E, erythroblasts, and erythrocytes. Blood 66:522-526, 1985.
USBio References
No references available
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