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169533 Rabbit Anti-HMG1 (HMG1, High Mobility Group 1) (Not for Export EU)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Isotype
IgG
Grade
Serum
Applications
WB
Crossreactivity
Rb
Shipping Temp
Blue Ice
Storage Temp
-20°C

High mobility group 1 (HMG1) is a 26kD highly con- served non-sequence-specific DNA-binding nuclear protein. Mammalian HMG1 has two homolo-gous DNA- binding domains HMG boxes, A and B (each of 80–90 amino-acid residues), linked by a short basic region to an acidic C-terminal domain containing 30 consecutive Asp and Glu residues. HMG1 has been implicated in a number of fundamental biological processes including transcription, replication and recombination, in which it plays a role in manipulating DNA structure by bending, looping, compaction or unwinding, or by direct contacts with distinct cellular proteins. HMG1 can act as a repressor, by interacting with TBP to block pre-initiation complex formation or as an activator, by facilitating the binding of various transcription factors to their cognate DNA sequences. Most recently, it was discovered that HMG1 is a late mediator of delayed endotoxin lethality by activating downstream cytokine release.

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:2000-1:5000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Peptide corresponding to a-HMG1.
Form
Supplied as a liquid.|
Purity
Serum.
Specificity
Recognizes HMG1. Reacts with the HMG1 protein in HeLa nu-clear extract
References
1. Yang et al. (2001) HMG-1 re-discovered as a cytokine. Shock 15:247. 2. Lu et al. (2000) Influence of HMG-1 and adenovirus oncoprotein E1A on early stages of transcriptional preinitiation complex assembly. J. Biol. Chem. 10:35006. 3. Jayaraman et al. (1998) High mobility group protein-1 (HMG-1) is a unique activator of p53. Genes Dev. 12:462. 4. Zlatanova et al. (1998) Binding to four-way junction DNA: a common property of architectural proteins? FASEB J. 12:421. 5. Zappavigna et al. (1996) HMG1 interacts with HOX proteins and enhances their DNA binding and transcriptional activation. EMBO J. 15:4981. 6. Ge et al. (1994) The high mobility group protein HMG1 can reversibly inhibit class II gene transcription by interaction with the TATA-binding protein. J. Biol. Chem. 269:17136. 7. Onate et al. (1994) The DNA-bending protein HMG-1 enhances progesterone receptor binding to its target DNA sequences. Mol. Cell. Biol. 14:3376. 8. Stelzer et al. (1994) Basal repression by HMG2 is counteracted by TFIIH-associated factors in an ATP-dependent process. Mol. Cell. Biol. 14:4712. 9. Bustin et al. (1990) Structural features of the HMG chromosomal proteins and their genes. Biochem. Biophys. Acta 1049:231.
USBio References
No references available
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